Cerca insegnamenti o docenti



FROM 3D-CULTURE AND 3D-PRINTING TO ORGANOIDS - Syllabus
FROM 3D-CULTURE AND 3D-PRINTING TO ORGANOIDS - Syllabus

Print / Stampa pagina

FULVIO GANDOLFI (responsabile dell'insegnamento)

CdL in SCIENZE BIOTECNOLOGICHE VETERINARIE (Classe LM-9) immatricolati dal 2016/17 - Laurea Magistrale - 2017/2018

Insegnamento obbligatorio / Compulsory courseNo
Anno di corso / Year of course2s
Periodo di svolgimentosecondo / second trimestre
Settori scientifico disciplinari / Scientific fields
  • VET/01 - Anatomia degli animali domestici (3 cfu: / ects: )
  • VET/09 - Clinica chirurgica veterinaria (2 cfu: / ects: )
  • AGR/18 - Nutrizione e alimentazione animale (3 cfu: / ects: )
Crediti (CFU) obbligatori / ECTS credits (CFU) compulsory8
Crediti (CFU) facoltativi / ECTS credits - facultative-

Informazioni generali / general information

Obiettivi: Cells in vivo are in a three-dimensional environment. Cells cultured in 3D models have biophysical characteristic and biomechanical signals, which more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The objective of the course is to give a comprehensive review of the main aspects and methods for 3D cell models from 3D-culture and 3d-printing to organoids.

Lingua dell'insegnamento / Language of instruction: English

Metodi didattici /Activities: Classical lectures, Laboratory direct interactions that will be integrated by webinars and on-line activities

Programma di studio / Syllabus

Short description english flag

Creating a 3D for cell culture is a relevant issue, but requires a multidisciplinary approach and multidisciplinary expertise. When entering the 3D, investigators need to consider the design of scaffolds for supporting the organisation of cells or the use of bioreactors for controlling nutrient and waste product exchange. Advances in materials chemistry, materials fabrication and processing technologies, and developmental biology have led to the design of 3D cell culture matrices that better represent the geometry, chemistry, and signaling environment of natural extracellular matrix. The intention of this course is to provide a general overview of the common approaches and techniques for designing 3D culture models. Three-dimensional printing represents the direct fabrication of parts layer-by-layer, guided by digital information. Over the past three decades, a variety of 3D printing technologies have evolved and has led to 3D bioprinting that allows the creation of biological constructs. This typically involves the use of cells dispensed onto a biocompatible surface using a successive layer-by-layer approach to generate tissue-like three-dimensional structures. The course will describe the main results obtained with this approach like skin tissues, heart tissues and blood vessels, and that contributed to significant advances in the medical field of biotechnology and tissue engineering. A part of the teaching will focus on the recent evolution of the technology that has allowed the creation of organs/organoids for transplant and for body on a chip as well as for tailor-made implants for patients.

Programma / Syllabus Module: 3 D approach to cell culture:

Cells cultured in 3D-model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D-monolayer of cells grown on standard plastic plates. Moreover, cells grow in a physiologically relevant environment, yet providing a challenge for assay chemistries originally designed for measuring events from monolayers of cells. 3D-culture methods depends on a number of factors, including the tissue you need to model and the particular research question you wish to explore. There is an unmet need for guidelines for design and validation of fit to purpose and effective assays useful for 3D-model systems. The course will provide the student with the understanding of the main aspects related to 3D-culture. Recommendations for factors to consider when verifying performance of cell health assays on 3D-culture models will also be presented.

Main topics:
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture

Programma / Syllabus Module: 3 D approach to cell culture per non frequentanti / not attendant students

Cells cultured in 3D-model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D-monolayer of cells grown on standard plastic plates. Moreover, cells grow in a physiologically relevant environment, yet providing a challenge for assay chemistries originally designed for measuring events from monolayers of cells. 3D-culture methods depends on a number of factors, including the tissue you need to model and the particular research question you wish to explore. There is an unmet need for guidelines for design and validation of fit to purpose and effective assays useful for 3D-model systems. The course will provide the student with the understanding of the main aspects related to 3D-culture. Recommendations for factors to consider when verifying performance of cell health assays on 3D-culture models will also be presented.

Main topics:
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture

Bibliografia e altri materiali didattici / Readings Module: 3 D approach to cell culture:

3D Cell Culture Methods and Protocols. Editors: Haycock, John (Ed.). Humana press. 2011.
Selected review articles, web-sites and webinars

Bibliografia e altri materiali didattici / Readings Module: 3 D approach to cell culture per non frequentanti / not attendant students

3D Cell Culture Methods and Protocols. Editors: Haycock, John (Ed.). Humana press. 2011.
Selected review articles, web-sites and webinars

Programma / Syllabus Module: 3 D bioprinting and practicals:

3D-bioprinters: philosophy and application in medicine
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid

Bibliografia e altri materiali didattici / Readings Module: 3 D bioprinting and practicals:

Selected review articles, web-sites and webinars

Programma / Syllabus Module: Cell interaction and organoids:

Mechano-sensing
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding

Bibliografia e altri materiali didattici / Readings Module: Cell interaction and organoids:

Selected review articles, web-sites and webinars

Modalità di esame, prerequisiti, esami propedeutici / Prerequisites, exams and assessment

Esame / Examunico
Modalità di accertamento conoscenze / Type of assessmentEsame
Giudiziovoto verbalizzato in trentesimi

Prerequisiti e modalità di esame Oral test

Oral test

Organizzazione didattica / Structure of the course

Module: 3 D approach to cell culture

obbligatorio / compulsory

Settori e relativi crediti / Scientific fields

  • AGR/18 - Nutrizione e alimentazione animale - cfu: / ects: 3
Attività didattiche previste / Learning activities

Lezioni: 18 ore / hours

Docenti / Teachers

Module: 3 D bioprinting and practicals

obbligatorio / compulsory

Settori e relativi crediti / Scientific fields

  • VET/09 - Clinica chirurgica veterinaria - cfu: / ects: 2
Attività didattiche previste / Learning activities

Lezioni: 12 ore / hours

Docenti / Teachers

Module: Cell interaction and organoids

obbligatorio / compulsory

Settori e relativi crediti / Scientific fields

  • VET/01 - Anatomia degli animali domestici - cfu: / ects: 3
Attività didattiche previste / Learning activities

Lezioni: 18 ore / hours

Ricevimento docenti / Teacher's office hours

Orario di ricevimento Docenti / Teacher's office hours
Docente / TeacherOrario di ricevimento / Office's hoursLuogo di ricevimento / Office location
FULVIO GANDOLFI (responsabile dell'insegnamento)Dal Lunedì al Venerdì su appuntamentoStudio
FABIO ACOCELLAappuntamento via mail
FEDERICA CHELIprevio appuntamentoSede di Via Trentacoste, 2

Altre informazioni / Further information

Detailed information on the programme are reported in the specific sections of the modules.